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i scei endonuclease expression vector  (Addgene inc)


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    Structured Review

    Addgene inc i scei endonuclease expression vector
    I Scei Endonuclease Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/i+scei+endonuclease+expression+vector+pcbascei/pCBASceI+(Plasmid+%2326477)/pm41611070-71-6-10
    Average 96 stars, based on 297 article reviews
    i scei endonuclease expression vector - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Transfection:

    Article Title: Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors
    Article Snippet: Anti-RAP80 was from Novus Biochemicals (Littleton, CO). .. HRR Assays SKOV-3-DR-GFP cells were transiently transfected with the empty vector pcDNA/Neo (Life Technologies) or the I-SceI endonuclease expression vector pCBASceI (Addgene plasmid 26477; Addgene) ( 25 ) using the TransFast reagent (Promega) according to the manufacturer’s protocol. .. In addition, SKOV-3 cells were transiently transfected with the EGFP expression vector pEGFP-N1 (Clontech Laboratories; Mountain View, CA) for monitoring the levels of GFP-positive cells to ensure that the expression of I-SceI was not affected by triapine treatment.

    Article Title: In silico screening identifies a novel small molecule inhibitor that counteracts PARP inhibitor resistance in ovarian cancer
    Article Snippet: SKOV3-EJ5-GFP cells were established by stable transfection of SKOV3 cells with pimEJ5-GFP plasmid (Addgene, Watertown, MA). .. Cells were transiently transfected with the empty vector pRc/CMV/Neo (Thermo Fisher Scientific) or the I-SceI endonuclease expression vector pCBASceI (Addgene) using Lipofactamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Two hr after transfection, cells were treated with various concentrations of DB4 for 48 h. Cells were then trypsinized, stained with 7-AAD, and analyzed for the percentage of GFP-positive and 7-AAD-positive cells by flow cytometry using an LSR II flow cytometer (BD Biosciences) and FlowJo software (Tree Star; Ashland, OR).

    Article Title: Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors
    Article Snippet: Anti-RAP80 was from Novus Biochemicals (Littleton, CO). .. SKOV-3-DR-GFP cells were transiently transfected with the empty vector pcDNA/Neo (Life Technologies) or the I-SceI endonuclease expression vector pCBASceI (Addgene plasmid 26477; Addgene) ( 25 ) using the TransFast reagent (Promega) according to the manufacturer’s protocol. .. In addition, SKOV-3 cells were transiently transfected with the EGFP expression vector pEGFP-N1 (Clontech Laboratories; Mountain View, CA) for monitoring the levels of GFP-positive cells to ensure that the expression of I-SceI was not affected by triapine treatment.

    Expressing:

    Article Title: Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors
    Article Snippet: Anti-RAP80 was from Novus Biochemicals (Littleton, CO). .. HRR Assays SKOV-3-DR-GFP cells were transiently transfected with the empty vector pcDNA/Neo (Life Technologies) or the I-SceI endonuclease expression vector pCBASceI (Addgene plasmid 26477; Addgene) ( 25 ) using the TransFast reagent (Promega) according to the manufacturer’s protocol. .. In addition, SKOV-3 cells were transiently transfected with the EGFP expression vector pEGFP-N1 (Clontech Laboratories; Mountain View, CA) for monitoring the levels of GFP-positive cells to ensure that the expression of I-SceI was not affected by triapine treatment.

    Article Title: In silico screening identifies a novel small molecule inhibitor that counteracts PARP inhibitor resistance in ovarian cancer
    Article Snippet: SKOV3-EJ5-GFP cells were established by stable transfection of SKOV3 cells with pimEJ5-GFP plasmid (Addgene, Watertown, MA). .. Cells were transiently transfected with the empty vector pRc/CMV/Neo (Thermo Fisher Scientific) or the I-SceI endonuclease expression vector pCBASceI (Addgene) using Lipofactamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s protocol. .. Two hr after transfection, cells were treated with various concentrations of DB4 for 48 h. Cells were then trypsinized, stained with 7-AAD, and analyzed for the percentage of GFP-positive and 7-AAD-positive cells by flow cytometry using an LSR II flow cytometer (BD Biosciences) and FlowJo software (Tree Star; Ashland, OR).

    Article Title: Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors
    Article Snippet: Anti-RAP80 was from Novus Biochemicals (Littleton, CO). .. SKOV-3-DR-GFP cells were transiently transfected with the empty vector pcDNA/Neo (Life Technologies) or the I-SceI endonuclease expression vector pCBASceI (Addgene plasmid 26477; Addgene) ( 25 ) using the TransFast reagent (Promega) according to the manufacturer’s protocol. .. In addition, SKOV-3 cells were transiently transfected with the EGFP expression vector pEGFP-N1 (Clontech Laboratories; Mountain View, CA) for monitoring the levels of GFP-positive cells to ensure that the expression of I-SceI was not affected by triapine treatment.

    Plasmid Preparation:

    Article Title: Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors
    Article Snippet: Anti-RAP80 was from Novus Biochemicals (Littleton, CO). .. HRR Assays SKOV-3-DR-GFP cells were transiently transfected with the empty vector pcDNA/Neo (Life Technologies) or the I-SceI endonuclease expression vector pCBASceI (Addgene plasmid 26477; Addgene) ( 25 ) using the TransFast reagent (Promega) according to the manufacturer’s protocol. .. In addition, SKOV-3 cells were transiently transfected with the EGFP expression vector pEGFP-N1 (Clontech Laboratories; Mountain View, CA) for monitoring the levels of GFP-positive cells to ensure that the expression of I-SceI was not affected by triapine treatment.

    Article Title: Triapine Disrupts CtIP-mediated Homologous Recombination Repair and Sensitizes Ovarian Cancer Cells to PARP and Topoisomerase Inhibitors
    Article Snippet: Anti-RAP80 was from Novus Biochemicals (Littleton, CO). .. SKOV-3-DR-GFP cells were transiently transfected with the empty vector pcDNA/Neo (Life Technologies) or the I-SceI endonuclease expression vector pCBASceI (Addgene plasmid 26477; Addgene) ( 25 ) using the TransFast reagent (Promega) according to the manufacturer’s protocol. .. In addition, SKOV-3 cells were transiently transfected with the EGFP expression vector pEGFP-N1 (Clontech Laboratories; Mountain View, CA) for monitoring the levels of GFP-positive cells to ensure that the expression of I-SceI was not affected by triapine treatment.



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